AKANSHA PATIL
Boston,MA
Overview
3
3
years of professional experience
Work History
Research
Nanomedicine Capstone
01.2025 - 04.2025
- Conducted comprehensive research to create a Theranostic probe against cancer (leukemia) cells.
- Executed series of experiments with HPLC, dialysis, mammalian cell culture, TEM, MRI.
- Used Doxorubicin and dextran-coated iron oxide nanoparticles to make drug nanoparticle conjugate.
- Compared the drug nanoparticle conjugate in two different pH mimicking lysosomal (4.7) and blood plasma (7.4) environment via dialysis.
- Analyzed the results drawing a drug release curve at both pH using HPLC.
Organic Chemistry Co-Op, Synthesis and Formulation, Research and Development
E Ink Corporation
01.2024 - 06.2024
- Conducted experiments to analyze Zeta potential and CPS (particle size) of raw pigment dispersions with charge control agents.
- Ran samples on 1H NMR and analyzing the results by determining chemical structure and composition.
- Analyzed results using the graph created by 1H NMR to check for carbon, hydrogen, and other chemical compounds to make the desired mixture.
Biotechnology Applications Laboratory
Northeastern University
01.2024 - 04.2024
- Conducted molecular biology experiments including pGLO transformation of E. coli to express GFP with the assistance of pGLO plasmid, and CRISPR-mediated gene editing of lacZ in E. coli to assess target gene expression
- Carried out biochemical assays like BCA assay, performed SDS-PAGE, GFP protein extraction from E. coli frozen pellets via Hydrophobic Interaction Chromatography (HIC) and AKTA-FPLC
- Performed BLAST (blastp) and FASTA by isolating 20-mer protein sequence of gene and producing DNA sequence, forward and reverse primers, multiple sequence alignment, visualizing it via PyMol / AlphaFold
- Validated immuno-assay western blot assay and ponceau staining by gel electrophoresis
Thesis Intern In-Vitro Cell Culture and Microbiology
Dr. D.Y. Patil Medical College and Hospital
02.2022 - 06.2022
- Familiarized with data collection, medical terminology, informed consent, patient requisition form, laboratory test form, etc.
- Performed blood sampling, streaking, incubation, isolation of microbial colonies, etc.
- Trained in applying biochemical assays like catalase, coagulase, urease, citrate to the infected samples to identify the infection causing bacteria, staining methods like gram stain and acid-fast stain, anti-microbial assay, analyzing the results via microscopy.
- Paid visits to different wards and ICUs of hospital to examine the on-site condition of CRBSI patients.
- Studied about CRBSI and calculated the number CRBSI cases from the total number of infections occurred which were around 50 out of 2000.
Education
Master of Science - Biotechnology
Northeastern University
Boston, MA12-2025
Bachelor in Technology - Biotechnology (B. Tech Biotechnology)
Dr. D.Y. Patil University of Biotechnology And Bioinformatics Institute
Pune, India07.2022
Skills
- Laboratory Skills: Analytical Chemistry, In-Vitro Assays, Sample Preparation, Gel Electrophoresis, HPLC, AKTA-FPLC, Pipetting, Mammalian Cell Culture, PCR, Cell Staining, Microscopy, NMR, Data Analysis
- Research Instruments: Centrifuge, Ultracentrifuge, 1H NMR, Rotavapor, BacT System
- Databases and Software: BLAST, Pymol, NCBI, PubMed, Biovia Workbook, MNova, MestreLab, Microsoft Suite
- Interpersonal Skills: Time Management, Attention to Detail, Communication, Teamwork, Adaptable
Timeline
Research
Nanomedicine Capstone
01.2025 - 04.2025
Organic Chemistry Co-Op, Synthesis and Formulation, Research and Development
E Ink Corporation
01.2024 - 06.2024
Biotechnology Applications Laboratory
Northeastern University
01.2024 - 04.2024
Thesis Intern In-Vitro Cell Culture and Microbiology
Dr. D.Y. Patil Medical College and Hospital
02.2022 - 06.2022
Master of Science - Biotechnology
Northeastern University
Bachelor in Technology - Biotechnology (B. Tech Biotechnology)
Dr. D.Y. Patil University of Biotechnology And Bioinformatics Institute
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