Calvin Yang
Watertown,ME
Summary
Diligent and adaptable scientist with exceptional memory and quick learning skills capable of executing tasks for multiples projects. Works well both in teams supporting senior colleagues as well as independently. Though work experiences may not align with the position, is already deeply familiar with the clinical context of ACR-368 and ACR-2316 as well as Acrivon's differentiating platform of a predictive phosphoproteomic biomarker. Meticulously sees projects through to completion, and is constantly anticipating needs or potential shortcomings.
Overview
4
4
years of professional experience
Work History
Senior Research Associate in Biomarker Development
Acrivon Therapeutics
Watertown, MA
06.2023 - Current
- Developed imaging-based assays in IF and IHC staining of cancer cell lines and tissues as an arm of the precision oncology predictive biomarker
- Used AI-based image analysis software to quantify tumors and cancer cells for protein biomarkers
- Conducted both manual and automated staining procedures
- Culture of cancer cell lines and generation cell pellet tissue microarray (cpTMA)
- Data processing and consolidation to evaluate assay reproducibility and efficacy
- Implemented biomarker assays to evaluate new indications for pipeline drugs ACR-368 and ACR-2316, including glioblastoma and head & neck as well as combination therapies such as immune checkpoint inhibitors and radiation therapy
- Performed cell viability assays and live-cell imaging to evaluate in vitro sensitivity of cancer lines to pipeline drugs
- Performed siRNA knockdown and Western blot to elucidate target engagement and antibody specificity
- Recordkeeping, inventory, and tracking of various antibodies for evaluation of biomarker candidates for predictive and pharmacodynamic applications
Associate Scientist II in Upstream PD/Cell Line Development/CMC
Dragonfly Therapeutics
Waltham, MA
02.2023 - 04.2023
- Stable cell line development/evaluation for proprietary tri-specific natural killer (NK) cell engager therapies (TriNKETs), multi-specific antibodies capable of facilitating antibody-dependent cellular cytotoxicity for oncological indications
- Transient lipid-based transfections, and stable electroporation-based transfections
- Culture of multiple suspension CHO lines and harvest for purification teams
- Molecular cloning and sequence analysis
- Fed-batch culture
- Ambr 250 bioreactor sampling and nutrient/metabolite analysis
Research Associate
Yurogen Biosystems
Worcester, MA
11.2021 - 02.2023
- Custom CRO monoclonal antibody development (rabbit/llama) for research insititutions and biotech
- Molecular cloning via linear expression module and DNA/RNA purification
- Mammalian cell culture and transfection (293T) for antibody testing
- Single B-cell culture in 96-well plate format
- Project management and progress reports for clients of ELISA titers
- SOP write-up, cost-accounting, and inventory keeping
Education
Master of Science - Physiology
Georgetown University Biomedical Graduate Education
Washington, D.C.06.2020
Bachelor of Arts - Molecular and Cellular Biology
Vanderbilt University College of Arts and Sciences
Nashville, TN05.2018
Skills
- H&E, IHC, and IF staining (manual and automated)
- Florescence and confocal imaging
- Molecular biology techniques
- Tissue collection, dissection and FFPE processing
- Mammalian cell culture
- Cell transfection (lipid and electroporation, DNA and RNA)
- Cell pellet and Tissue Microarray production
- SDS-PAGE and Western Blot
- ELISA
- Viability Assay (CTG)
- Live Cell Imaging
- Hardware: Leica Bond, CellCyte, Tecan Digital Dispenser
- Communication
- Teamwork
- Adaptability
- Attention to Detail
- Software: Prism, Microsoft Office, Benchling, LabFolder, HALO
Timeline
Senior Research Associate in Biomarker Development
Acrivon Therapeutics
06.2023 - Current
Associate Scientist II in Upstream PD/Cell Line Development/CMC
Dragonfly Therapeutics
02.2023 - 04.2023
Research Associate
Yurogen Biosystems
11.2021 - 02.2023
Bachelor of Arts - Molecular and Cellular Biology
Vanderbilt University College of Arts and Sciences
Master of Science - Physiology
Georgetown University Biomedical Graduate Education
Publications
Duffy, D. J., Schnitzler, C., Karpinski, L., Thomas, R., Whilde, J., Eastman, C., Yang, C., Krstic, A., Rollinson, D., Zirkelbach, B., Yetsko, K., Burkhalter, B., & Martindale, M. Q. (2018).
Sea turtle fibropapilloma tumors share genomic drivers and therapeutic vulnerabilities with human cancers.
Communications Biology, 1(1), 63. https://doi.org/10.1038/s42003-018-0059-x
Whilde, J., Whitmore, L., Yang, C., Eastman, C. B., Thomas, R., Rollinson, D., Burkhalter, B., Martindale, M. Q., The Whitney Laboratory for Marine Bioscience & Sea Turtle Hospital, Duffy, D. J., The Whitney Laboratory for Marine Bioscience & Sea Turtle Hospital, & Department of Biological Sciences, School of Natural Sciences, Faculty of Science and Engineering, University of Limerick, Limerick, Ireland. (2019).
Behaviour of juvenile green turtles (Chelonia mydas) before and after fibropapillomatosis tumour removal.
In Testudo: Vol. Vol. 9 (Issue No. 1) [Journal-article].