Grace Trombley

• Established macrophage/tumor cell coculture phagocytosis platform
• Designed and executed genome-wide CRISPR screens to discover novel targets enhancing macrophage phagocytosis of tumor cells
• ·Executed target validation and MOA studies on tumor intrinsic targets utilizing functional assays.
• Implemented protocol for monocyte isolation from PBMCs and subsequent differentiation into macrophages.
• Developed Perturb-seq to enhance existing screening platforms for identifying novel potential IE contexts of clinical relevance
• Guided and developed junior scientists
• Interpreted data and routinely communicated results to senior leadership teams

• Established and optimized standardized protocol on flow cytometry staining and acquisition/analysis
• Regularly conducted flow cytometry to evaluate biomarkers and monitor cell viability at essential points in sc-islet differentiation.
• Acquired and processed samples for IFC analysis in both 2D and Whole Mount modalities
• Executed Glucose Stimulated Insulin Secretion assay on late stage islets alongside performing ELISA on samples gathered from GSIS.
• Executed In-Vivo insulin injection administration daily and conducted glucose measurements
• Managed IPS cell culture maintenance while advancing differentiation processes into Beta Islet cells in 2D and 3D environments

• Executed various cell based assays, specifically ELISA and Flow Cytometry
• Maintained multiple mammalian cell lines
• Formulated SOPs and protocols to enhance assay development for client projects.
• Generated comprehensive data overviews and completed reports for client delivery
• Oversee laboratory maintenance of tissue culture facility, ensuring preparation of reagents and upkeep of equipment.

Examined the regulation of Notch1 in differentiated T cells by inhibiting PKCq function

• Extracted CD4 T cells from mouse splenocytes and cultivated T cells under polarizing conditions into various T cell lineages.
• Evaluated expression profiles of pPKCq, Notch1C, and various transcription factors through flow cytometry.
• Leveraged AMNIS imaging flow cytometry for identification of protein localization

• Annotated TCR loci of cynomolgus macaque through comparison of germline sequence homology between humans and Rhesus monkeys
• Validated annotations through PCR and sequencing

Tested the hypothesis that FANCJ coordinates effective DNA repair processing and inhibits competing pathways

• Generated site-directed UV lesions in DNA and performed immunofluorescence experiments to evaluate accumulation of RAD51 in FANCJ-sufficient and –deficient HeLa cells
• Evaluated presence of RPA in FANCJ-deficient HeLa cells, establishing reduced levels at sites of UV-induced DNA damage