Isabela De Oliveira
Boston
Summary
Research associate with proven track record in enhancing knowledge bases through comprehensive analysis and problem-solving expertise. Demonstrated ability to deliver actionable insights and drive project success.
Overview
3
3
years of professional experience
Work History
Research Associate - Analytical Development (Contract)
Vertex Pharmaceuticals
Boston
08.2025 - Current
- Performed manual and automated cell culture of primary cells, stem cells, and mammalian lines for cell therapy programs.
- Executed cell-based assays (viability, fluorescence microscopy, automated flow cytometry, In vitro Differentiation (IVD), Colony Forming Unit (CFU))
- Maintained records using ELN and LIMS; collaborated via MS Teams and SharePoint.
- Managed lab inventory, followed GLP-like practices, and supported cross-functional data tasks.
Research Assistant
Harvard Medical School Church Lab
Boston
01.2023 - 05.2025
- Conducted independent research on T cell aging to understand mechanisms impacting immune response and disease outcomes.
- Designed and executed in vitro experiments using flow cytometry, PCR, and mammalian cell culture in a fast-paced research setting.
- Applied CRISPR-based gene editing to modulate transcription factor expression, including ZNF family proteins, in primary T cells.
- Utilized viral vector-based delivery systems to introduce gene-editing constructs into T cells.
- Analyzed and interpreted Flow Cytometry data using FlowJo; communicated results effectively within interdisciplinary research teams.
- Collaborated with Harkamal Jhajj and George Church for guidance and feedback.
Program for Research in Science and Engineering Fellow
06.2023 - 08.2023
- Completed 10-week fellowship focused on T-cell research.
- Validated transcription factor candidates to improve T-cell fitness.
- Optimized experimental conditions to ensure statistical significance of results.
Education
AB - Human Developmental & Regenerative Biology, Global Health & Health Policy
Harvard University
Cambridge, MA05.2025
Certificate of Completion - Fortaleza, Ceara, Brazil
Harvard-Brazil Collaborative Public Health Field Course
Fortaleza, Ceara, Brazil01.2025
Skills
- In vitro studies
- Cell culture
- Flow cytometry
- Data analysis
- Molecular biology assays
- Gene and protein analysis
- Cell-based assays
- Viability assessment
- Mammalian cell culture
- Transduction techniques
- Viral vector delivery
- Plasmid cloning
- CRISPR technology
- GraphPad Prism proficiency
- FlowJo expertise
- Electronic lab notebook documentation
- Benchling usage
- Experimental record keeping
Websites
Languages
- Portuguese (native)
- Spanish (proficient)
Languages
Portuguese
Native/ Bilingual
Spanish
Limited
Timeline
Research Associate - Analytical Development (Contract)
Vertex Pharmaceuticals
08.2025 - Current
Program for Research in Science and Engineering Fellow
06.2023 - 08.2023
Research Assistant
Harvard Medical School Church Lab
01.2023 - 05.2025
AB - Human Developmental & Regenerative Biology, Global Health & Health Policy
Harvard University
Certificate of Completion - Fortaleza, Ceara, Brazil
Harvard-Brazil Collaborative Public Health Field Course