Julie LePrevost
Watertown
Summary
Accomplished Research Scientist with extensive experience in the Biotechnology field, specializing in translational research and assay development. Proven expertise in flow cytometry and multiplex immunofluorescence, coupled with strong problem-solving skills. Successfully designed and executed in vivo studies, delivering impactful data presentations that advanced clinical goals.
Overview
6
6
years of professional experience
Work History
Research Scientist III
Werewolf Therapeutics
03.2024 - Current
- Company Overview: Clinical; Translational Sciences
- Explored translational research for the clinical team, developing assays utilizing human tumors; drug response in dissociated and tumor fragments ex vivo culture assays using flow cytometry, MSD cytokine assays, and ELISpot readouts
- 3D ex vivo human tumor fragment culturing using matrigel matrix
- Skilled user of Lunaphore Comet for multiplex immunofluorescence and Halo software for analysis; development of clinical biomarker and secondary endpoint assay, as well as preclinical mechanism of action studies
- Analysis of clinical trial patient biomarker flow cytometry and cytokine data, patient data tracking
- Clinical Translational team
Research Scientist III
Werewolf Therapeutics
01.2022 - 03.2024
- Company Overview: Translational Pharmacology team
- Extensive experience on Cytek Aurora spectral cytometer and FlowJo software, also with BD Fortessa. MSD QuickPlex SQ 120MM, Jess Simple Western, StepOnePlus PCR machine (RT-qPCR)
- Mechanism of action and efficacy in vivo studies; sample processing and flow cytometry analysis of immune components (including TILs) in multiple models with 25+ color panel on Cytek Aurora, blood, splenocyte and liver staining
- Experience with ELISpot and multiplex MSD assays with analysis
- Lentiviral production; validated in house generation of lentivirus using LV MAX system (DNA plasmid prep/purification), HEK cell lentiviral titering
- Lentiviral transduction of human T cells (third and fourth generation CART), HEK and Jurkat with transduction efficacy testing (flow cytometry, RT-qPCR with experience designing primers based off construct design) and cytotoxicity testing and assay development (flow cytometry and cytokine ready outs)
- In vitro assay development, PK ELISAs and analysis
- Developing Flow cytometry panels >10 colors using Cytek Aurora
- Cell culture maintenance and cell based assays; HEK, Jurkat, Raji, Daudi, Nalm-6, mEER, human T cells and PBMCs
- Experience writing In vivo study reports for IND, presenting data regularly, striving to meet company goals within deadlines
- Translational Pharmacology team
Research Scientist II
Werewolf Therapeutics
10.2021 - 01.2022
- Company Overview: Translational Pharmacology team
- Extensive experience on Cytek Aurora spectral cytometer and FlowJo software, also with BD Fortessa. MSD QuickPlex SQ 120MM, Jess Simple Western, StepOnePlus PCR machine (RT-qPCR)
- Mechanism of action and efficacy in vivo studies; sample processing and flow cytometry analysis of immune components (including TILs) in multiple models with 25+ color panel on Cytek Aurora, blood, splenocyte and liver staining
- Experience with ELISpot and multiplex MSD assays with analysis
- Lentiviral production; validated in house generation of lentivirus using LV MAX system (DNA plasmid prep/purification), HEK cell lentiviral titering
- Lentiviral transduction of human T cells (third and fourth generation CART), HEK and Jurkat with transduction efficacy testing (flow cytometry, RT-qPCR with experience designing primers based off construct design) and cytotoxicity testing and assay development (flow cytometry and cytokine ready outs)
- In vitro assay development, PK ELISAs and analysis
- Developing Flow cytometry panels >10 colors using Cytek Aurora
- Cell culture maintenance and cell based assays; HEK, Jurkat, Raji, Daudi, Nalm-6, mEER, human T cells and PBMCs
- Experience writing In vivo study reports for IND, presenting data regularly, striving to meet company goals within deadlines
- Translational Pharmacology team
Research Associate I: Biology
Selecta Biosciences Inc.
07.2019 - 10.2021
- Responsible for in vivo experimental study design
- Executing in vivo experiments pertaining to mechanism of action studies and disease indications (mouse models)
- Mouse handling; blood collection, injection (I.P., R.O.), gavage, organ collection (for single cell suspensions and OCT block cryofreezing)
- Immune cell isolation from murine livers and splenocytes for flow cytometry (work with BD Canto 2, FACS Diva software) cell characterization; multi-color cell surface and intracellular staining
- Flow cytometry data analysis with FlowJo software, experience with 8 colors in one panel
- In vitro and ex vivo assay development; proliferation/suppression assays and cytokine analysis (MSD)
- Sterile cell culture technique; passage primary cell lines, manual cell counting, counting via cell countess and Vi-cell
- Met progressive deadlines to provide phenotypic and functional data for the publication shown above, as well as for many other projects in collaboration with the biology team and the translational biology and bioanalytical team to meet company goals
- Maintained laboratory notebooks, regularly presented data and journal clubs in meetings
- Regular use; FlowJo, FACSDiva, GraphPad Prism, Microsoft Excel and Word
Education
Graduate Immunology -
Harvard Extension School
01.2020
AAI Advanced Immunology Seminar -
American Association of Immunology
01.2020
M.S. of Health Sciences - Biomedical Sciences
Quinnipiac University
Hamden, CT05.2019
B.S. - Biomedical Sciences
Quinnipiac University
Hamden, CT05.2018
Skills
- Translational research
- Flow cytometry
- Assay development
- Multiplex Immunofluorescence
- Problem solving
- Attention to detail
- Experimental design
- Data presentation
Publications
Enhancement of the Tolerogenic Phenotype in the Liver by ImmTOR Nanoparticles, Frontiers in Immunology, 05/25/21
Timeline
Research Scientist III
Werewolf Therapeutics
03.2024 - Current
Research Scientist III
Werewolf Therapeutics
01.2022 - 03.2024
Research Scientist II
Werewolf Therapeutics
10.2021 - 01.2022
Research Associate I: Biology
Selecta Biosciences Inc.
07.2019 - 10.2021
Graduate Immunology -
Harvard Extension School
AAI Advanced Immunology Seminar -
American Association of Immunology
M.S. of Health Sciences - Biomedical Sciences
Quinnipiac University
B.S. - Biomedical Sciences
Quinnipiac University
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