MAHIMA GUPTA

Introduction to Biology

Microbiology

Genetics

• Helped with grading assignments and tests, providing constructive feedback to students based on results.
• Maintained laboratory safety by demonstrating equipment and techniques.
• Worked with teacher to optimize learning plans based on student test grades and evaluations.

Project title: In-vitro molecular characterization of p53 gene in Genomic DNA extracted from formaldehyde-fixed tissues,
Extracted DNA from formaldehyde-fixed tissue samples of cancer patients and analyzed p53 gene mutation in carcinogenic conditions
DNA extraction was done manually, and molecular characterization of p53 gene was done using PCR

Project title: Molecular Diagnosis of Philadelphia Chromosome negative Myeloproliferative Neoplasms (MPNs).
Extracted DNA from blood samples of cancer patients for molecular detection of somatic valine to phenylalanine (V617F) mutation on JAK-2 associated with MPNs for further treatment of the disease.
JAK-2 (V617F) mutation was detected in extracted DNA samples by amplification refractory mutation system PCR (ARMS-PCR) for progression and prognosis of the disease.

Project title: An insight into the Behavioral Paradigms of Cognition

Performed experiments on mouse models under physiological and pathological conditions to approximate human behavioral responses and measure anxiety-like behaviors, spatial memory and learning, decision-making, and assessing work memory, to map affected brain regions by comparable mechanisms in humans and mice.