Nicholina Love Allain
Leominster
Summary
Self-motivated Process Development Research Associate with 4+ years experience in optimization and scale-up of pre-clinical cell therapy products. Competency in basic and specialized laboratory techniques, instrumentation, and data analysis software. Strong collaborator experienced in regulatory compliance and GMP equipment onboarding. Dedicated team player with demonstrated success in cell culture techniques and analytical assay execution.
Overview
8
8
years of professional experience
1
1
Certification
Work History
Research Associate II, Process Development
RoslinCT Boston
Hopkinton, MA
03.2024 - Current
- Performed solid tumor dissections to isolate and expand tumor-infiltrating lymphocytes (TILs) for adoptive cell therapy in solid tumor biopsies.
- Assisted in process optimization and scale-up techniques for in-house TIL rapid expansion protocols.
- Executed successful iPSC 3D scale-up and expansion protocols using small-scale vertical-wheel bioreactors (0.1 L - 3 L).
- Lead successful execution of iPSC differentiation protocols to intermediate lymphoid progenitor cells and transition to final targeted NK cells.
- Evaluated novel mechanoporation and novel final formulation instruments for market readiness, regulatory compliance and assisted with creation of business cases to strengthen company process innovation capabilities via procurement.
- Experience with maintenance, expansion, and banking of in-house and externally sourced healthy control stem cell lines using different media formulations, basement membranes, and passaging techniques.
- Experience with flow cytometry intra/extracellular staining procedures, data analysis/acquisition of iPSC, NK and other lymphoid cell panels.
- Execution of various sandwich ELISA cytokine quantification assays, instrument analysis and reporting to assist Analytical Development team.
Research Associate I, Process Development
RoslinCT Boston
Hopkinton, MA
02.2022 - 03.2024
- Performed solid tumor dissections to isolate and expand tumor-infiltrating lymphocytes (TILs) for adoptive cell therapy in solid tumor biopsies.
- Assisted in process optimization and scale-up techniques for in-house TIL rapid expansion protocols.
- Executed successful iPSC 3D scale-up and expansion protocols using small-scale vertical-wheel bioreactors (0.1 L - 3 L).
- Lead successful execution of iPSC differentiation protocols to intermediate lymphoid progenitor cells and transition to final targeted NK cells.
- Evaluated novel mechanoporation and novel final formulation instruments for market readiness, regulatory compliance and assisted with creation of business cases to strengthen company process innovation capabilities via procurement.
- Experience with maintenance, expansion, and banking of in-house and externally sourced healthy control stem cell lines using different media formulations, basement membranes, and passaging techniques.
- Experience with flow cytometry intra/extracellular staining procedures, data analysis/acquisition of iPSC, NK and other lymphoid cell panels.
- Execution of various sandwich ELISA cytokine quantification assays, instrument analysis and reporting to assist Analytical Development team.
- Lead build-out of novel 1,700 ft² process development lab with procurement/onboarding of 100+ RUO equipment assets, essential furnishings and consumables for laboratory functionality.
- Lead successful scale-up of autologous neoantigen T cell therapy for client technology transfer to clinical setting.
- Assisted in successful demonstration of NK cell expansion in 2D culture conditions over several internal experiments.
Quality Control Analyst I, In-Process
Nitto Denko Avecia
Milford, MA
09.2021 - 02.2022
- Tested crude samples, fractions, and conducted mock-pool impurity testing on oligonucleotide products using mobile phase HPLC analysis.
- Introduction to/development of cGMP techniques in
- Experience with Waters HPLC instrumentation and data analysis using Empower 3 software.
Analytical Chemist I, Wet Chemistry
Alpha Analytical
Westborough, MA
09.2020 - 09.2021
- Lead multiple assays testing for various carcinogenic agents in soil, water, and other sample types located across the US Northeast Coast.
- Experienced execution on consecutive 8+ hour assays, LIMS software, and delivering results across multiple assays with minimal turnaround times.
- Experience with execution and data reporting of assays including hexavalent chromium extraction, cyanide and sulfide reactivity testing, cyanide distillation extraction, pH/ORP and alkalinity testing.
Research Lab Assistant, Department of Biology
Assumption University
Worcester, MA
09.2018 - 03.2019
- Assisted professor with small scale, independent collection of human hair samples for subsequent comparison of carbon and nitrogen isotope levels on the basis of diet.
- Experience with Institutional Review Board (IRB) regulations, data collection and preparation for isotope analysis, literature analysis and presentation of findings.
Education
Bachelor’s of Art - Biotechnology and Molecular Biology
Assumption University
Worcester, MA05-2019
Skills
- Benchling ELN Software
- 5S Workplace
- GraphPad Prism 10 Software
- Sandwich ELISA
- Image-Pro 11 Software
- GLP, cGMP
- Flow Cytometry Analysis (FACS Suite/FCS Express)
- BBP Certification
- Six Sigma Green Belt Candidate
- Aseptic Techniques
- Microsoft Suite (Word, Excel, etc)
- Cell Culture
- Apheresis
- Equipment Onboarding Lifecycle
- DNA Extraction
Certification
- Bloodborne Pathogens Certification (29 CFR 1910.1030)
- Certification ID: NOF-1755699945-3512-45403
- Expiration Date: 08/20/2026
Timeline
Research Associate II, Process Development
RoslinCT Boston
03.2024 - Current
Research Associate I, Process Development
RoslinCT Boston
02.2022 - 03.2024
Quality Control Analyst I, In-Process
Nitto Denko Avecia
09.2021 - 02.2022
Analytical Chemist I, Wet Chemistry
Alpha Analytical
09.2020 - 09.2021
Research Lab Assistant, Department of Biology
Assumption University
09.2018 - 03.2019
Bachelor’s of Art - Biotechnology and Molecular Biology
Assumption University