Trisha Das
Medford
Summary
Associate Scientist with over 5+ years of experience in biotechnology, specializing in CRISPR technology and high-throughput screening to enhance drug discovery processes. Expert in cell culture, data management, and cell-based functional assays, with a track record of leveraging automated instruments to drive genetic perturbation experiments to success. Passionate about applying innovative techniques to solve complex biological problems, aiming to contribute to groundbreaking advancements in gene editing.
Overview
8
8
years of professional experience
Work History
Senior Associate Scientist
Intellia Therapeutics Inc.
04.2019 - Current
- Former, Senior Research Associate (April, 2019 - April, 2021) and Associate Scientist (April, 2021 - July 2024).
- Conduct precise CRISPR screenings to nominate lead drug candidates targeting specific genes.
- Collaborate with cross-functional teams to develop .innovative gene editing tools and refine existing methods in novel cell systems.
- Optimize high throughput screening protocols, ensuring accuracy, precision, and consistency.
- Plan, execute, and deliver end-to-end experiments, establishing SOPs for streamlined production work.
- Maintain comprehensive documentation to ensure data integrity and compliance with regulatory standards.
Research Assistant
BioAffinity Technologies Inc.
07.2018 - 04.2019
- Former, Research Intern (January 2018-May, 2018).
- Lead efforts in developing effective anticancer therapies through RNAi-mediated knockdown of targeted genes
- Utilized molecular and cell-based assays to investigate non-invasive diagnostics and therapeutics, focusing on specific protein expression in various cancer cells
- Maintained primary and cancer cell lines, including media preparation, cryopreservation, cell counting, and growth curve analysis
- Develop functional assays to assess cell proliferation, viability, and toxicity, contributing to the evaluation of potential therapeutic interventions.
Graduate Research Assistant
University of Texas at San Antonio
06.2016 - 12.2017
- Conducted functional analysis of pheromone binding venom proteins in Solenopsis invicta using MALDI TOF mass spectrometry
- Proficient in tissue embedding and sectioning for MALDI TOF sample preparation
- Contributed to research efforts aimed at understanding the role of venom proteins in pheromone binding.
Graduate Teaching Assistant
University of Texas at San Antonio
08.2016 - 12.2016
- Responsibilities include teaching, organizing, and conducting the lab.
Education
MS in Biotechnology -
The University of Texas
San Antonio, TX05.2018
MS in Biomedical Technology -
Gujarat University
India 04.2015
BS in Biochemistry and Vocational Biotechnology -
St. Xaviers College
India 04.2013
Skills
- Cell culture (Cancer cell lines, Primary cells, T-Cells, IPSC derived cell lines)
- Cell-based Functional assays (CTG, LDH)
- Nucleic acid techniques (PCR, Digital PCR, DNA manipulation)
- Protein expression assays (MSD, ELISA, Western blots)
- NGS (DNA isolation, library preparation, sequencing, data analysis)
- Data analysis (Excel,GraphPad Prism, Python)
- Data management (Electronic Lab notebook)
- Project management ( JIRA)
- Automated instruments (TapeStation, Bioanalyzer, robotic liquid handlers – Hamilton STAR, Integra)
Publications
- Identification of a novel mechanism for meso‐tetra (4‐carboxyphenyl) porphyrin (TCPP) uptake in cancer cells, David J. Elzi, William E. Bauta, Jamila R. Sanchez, Trisha Das, Shweta Mogare, Peter Zannes Fatland, Moises Iza, Alexander Pertsemlidis, Vivienne I. Rebel, The FASEB Journal, 35(3), 02/2021
- Major venom proteins of the fire ant Solenopsis invicta (Hymenoptera: Formicidae): Functional insights from mass spectrometric analysis, Trisha Das, Ibrahim Alabi, Madeline Colley, Fangzhi Yan, Wendell Griffith, Stephan Bach, Susan T. Weintraub, Robert Renthal, Insect Molecular Biology, 27(4), 505-511, 04/2018
Timeline
Senior Associate Scientist
Intellia Therapeutics Inc.
04.2019 - Current
Research Assistant
BioAffinity Technologies Inc.
07.2018 - 04.2019
Graduate Teaching Assistant
University of Texas at San Antonio
08.2016 - 12.2016
Graduate Research Assistant
University of Texas at San Antonio
06.2016 - 12.2017
MS in Biotechnology -
The University of Texas
MS in Biomedical Technology -
Gujarat University
BS in Biochemistry and Vocational Biotechnology -
St. Xaviers College
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